The role of<i>CRP</i>and<i>ATG9B</i>expression in clear cell renal cell carcinoma

Abstract

The purpose of the study is to investigate the correlation between the expression of C-reactive protein (<i>CRP</i>) and autophagy-related 9B (<i>ATG9B</i>) and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of <i>CRP</i> and <i>ATG9B</i> on the apoptosis and cell cycle progression of CCRCC cell line. <i>ATG9B</i> expression in CCRCC tissues and adjacent renal tissues was analyzed by immunohistochemistry (IHC). Gene expression was determined at transcription and translational levels using real-time quantitative PCR (RT-qPCR) and Western blot. The association between <i>CRP/ATG9B</i> expression and clinical-pathological parameters including age, gender, pathological grades, TNM stage and distant metastasis of the patients was assessed by correlation analysis. siRNA and overexpression plasmids construction were used to manipulate the expression of <i>CRP</i> in human CCRCC cell line 786-O. Cell apoptosis and cell cycle progression were determined using flow cytometry (FCM) and Hoechst 33258 staining. <i>CRP</i> expression correlates with <i>ATG9B</i> expression. The expression of <i>CRP</i> and <i>ATG9B</i> are significantly correlated with TNM staging, distant metastasis, and survival time of CCRCC patients. A high-level of <i>CRP</i> indicates a poor overall survival (OS). In addition, <i>CRP</i> expression influences cell cycle and apoptosis of CCRCC cells. The study reveals that <i>CRP</i> might be a CCRCC development promoter. In addition, there is a close relationship between <i>CRP</i> and <i>ATG9B</i> in CCRCC carcinogenesis.