Rapid biosynthesis of<i>Bauhinia variegata</i>flower extract-mediated silver nanoparticles: an effective antioxidant scavenger and α-amylase inhibitor

Abstract

Silver nanoparticles (AgNPs) were biosynthesized using Bauhinia variegata flower extract (BVFE). The BVF-AgNPs was found to be spherical shaped with the size of 5-15 nm. The phytoconstituents analysis and FTIR spectrum indicated that bioactive compounds like, phenols, flavonoids, benzophenones, nitro compounds, aromatics and aliphatic amines from BVFE might absorb on the surface of BVF-AgNPs. The synthesized BVF-AgNPs showed potent antioxidant property and α-amylase enzyme activity inhibition. The IC₅₀ value of BVF-AgNPs was found to be 4.64 and 16.6 µg/ml for DPPH and ferric reducing power assay, respectively. The IC₅₀ value of BVF-AgNPs for α-amylase inhibition was found to be 38 µg/ml. The K_i value of BVF-AgNPs for α-amylase inhibitory effect was found to be 21 µg/ml with the non-competitive mode of inhibition. These results suggest that BVF-AgNPs might be an effective nano-drug to treat diabetic conditions.