Genetic Manipulation of the COP9 Signalosome Subunit PfCsnE Leads to the Discovery of Pestaloficins in <i>Pestalotiopsis fici</i>

Abstract

By deleting the COP9 signalosome subunit PfcsnE from Pestalotiopsis fici, seven compounds that were newly produced by the mutant could be characterized, including five new structures, pestaloficins A-E (1 and 3-6). Pestaloficin A (1) represents a new type of dimeric cyclohexanone derivative linked through an unprecedented pentacyclic spiral ring.