Direct uptake of HCO₃⁻ in the marine angiosperm <scp><i>Posidonia oceanica</i></scp> (L.) Delile driven by a plasma membrane H⁺ economy

Abstract

Seagrasses access HCO₃^- for photosynthesis by 2 mechanisms, apoplastic carbonic anhydrase-mediated dehydration of HCO₃^- to CO₂ and direct HCO₃^- uptake. Here, we have studied plasma membrane energization and the mechanism for HCO₃^- import in Posidonia oceanica. Classical electrophysiology and ion-selective microelectrodes were used to measure the membrane potential, cytosolic pH, and the cytosolic concentrations of Na⁺ and Cl^- upon the addition of HCO₃^- . The photosynthetic response to HCO₃^- and to inhibitors was also measured. Results indicate that the primary pump of P. oceanica plasma membrane is a fusicoccin-sensitive H⁺ -ATPase. Bicarbonate depolarizes the plasma membrane voltage and transiently acidifies the cytosol, indicating that HCO₃^- is transported into the cells by an H⁺ -symport. Initial cytosolic acidification is followed by an alkalinization, suggesting an internal dehydration of HCO₃^- . The lack of cytosolic Na⁺ and Cl^- responses rules out the contribution of these ions to HCO₃^- transport. The energetics of nH⁺ /HCO₃^- symport allows, for n = 1, an estimate of cytosolic accumulation of 0.22 mM HCO₃^- . Because this transporter could permit accumulation of HCO₃^- up to 100 times above the equilibrium concentration, it would be a significant component of a carbon-concentrating mechanism in this species.