Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

TLDR

The Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG) are extensions of the Minimum Information about Any (x) Sequence (MIxS) framework and can be used alongside other GSC checklists such as MIGS, MIMS, and MIMARKS. The Genomic Standards Consortium presents MISAG and MIMAG as new standards for reporting bacterial and archaeal genome sequences. MISAG and MIMAG specify requirements for assembly quality, genome completeness, contamination estimates, and other metadata. Adopting MISAG and MIMAG across the community will enable more robust comparative genomic analyses of bacterial and archaeal diversity.

Abstract

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.