Effects of low power violet laser irradiation on red blood cells volume and erythrocyte sedimentation rate in human blood

Abstract

This study is designed in vitro to examine the effects of low power violet laser irradiation on some human blood samples rheological factors such as mean red blood cell volume (MCV) and erythrocyte sedimentation rate (ESR). Blood samples were collected into EDTA contained tubes and separated into two equal aliquots to be attended as irradiated and control. Samples were irradiated for 20, 30, 40 or 50 min with a laser of power 10 mW. The measurements were done directly after irradiation by applying westergen method and using a computerized hemtoanalyzer. The RBCs volume and ESR were decreased after irradiation for 40min by 0.44% and 6.7% respectively. It is possible to suggest that laser irradiation can reduction red blood cells volume because of the increased concentrations of free intracellular Ca+². The result shows that ESR reduction exposed to low power laser is mostly by reason of the effect of laser on composition of the plasma that finally affects in ESR of whole blood.