Genome-Wide Identification and Transcriptional Expression Analysis of Cucumber Superoxide Dismutase (SOD) Family in Response to Various Abiotic Stresses

Abstract

Superoxide dismutase (SOD) proteins are widely present in the plant kingdom and play important roles in different biological processes. However, little is known about the <i>SOD</i> genes in cucumber. In this study, night <i>SOD</i> genes were identified from cucumber (<i>Cucumis sativus</i>) using bioinformatics-based methods, including 5 <i>Cu/ZnSOD</i>s, 3 <i>FeSODs</i>, and 1 <i>MnSOD</i>. Gene structure and motif analysis indicated that most of the <i>SOD</i> genes have relatively conserved exon/intron arrangement and motif composition. Phylogenetic analyses with SODs from cucumber and several other species revealed that these SOD proteins can be traced back to two ancestral SODs before the divergence of monocot and dicot plants. Many <i>cis</i>-elements related to stress responses and plant hormones were found in the promoter sequence of each <i>CsSOD</i> gene. Gene expression analysis revealed that most of the <i>CsSOD</i> genes are expressed in almost all the tested tissues. qRT-PCR analysis of 8 selected <i>CsSOD</i> genes showed that these genes could respond to heat, cold, osmotic, and salt stresses. Our results provide a basis for further functional research on <i>SOD</i> gene family in cucumber and facilitate their potential applications in the genetic improvement of cucumber.