Metabolic engineering of Escherichia coli for production of n-butanol from crude glycerol

Abstract

The approach by rational rewiring of metabolic pathways enables <i>E. coli</i> to synthesize n-butanol from glycerol in an efficient way. Our proposed strategies illustrate the feasibility of manipulating key metabolic nodes at the junction of the central catabolism. As a result, it renders the intracellular redox state adjustable for various purposes. Overall, the developed technology platform may be useful for the economic viability of the glycerol-related industry.