A chemical method for the quantitative determination of 2-methoxyoestrone, oestrone, ring D α-ketolic oestrogens, oestradiol-17β, 16-<i>epi</i>oestriol and oestriol in human urine

Abstract

The object of this investigation was to develop a method for the quantitative determination of 2-methoxyoestrone, oestrone, ring D x-ketolic oestrogens, oestradiol-17k, 16-epioestriol and oes- triol in human urine. The development of the method began with a detailed study of the experi- mental conditions required for quantitative separa- tion of pure ketonic and non-ketonic oestrogens by means of the Girard reaction and the development of partition columns for the separation of the oestrogens in each of the Girard fractions (Givner, 1959; Givner, Bauld & Vagi, 1960).