Development of efficient techniques for clonal multiplication of Jatropha curcas L., a potential biodiesel plant

Abstract

Effect of auxins (IAA, IBA and NAA) and vitamin B 1 (thiamine) on rooting response of branch cuttings and air-layers of Jatropha curcas during spring and monsoon seasons was studied. Spring season was found best for clonal multiplication of genetically superior material in jatropha. Cuttings treated with 600 and 800 mg l ―1 thiamine showed 100% sprouting during both seasons. The average sprout growth was also found maximum in thiamine treated cuttings. Auxins enhanced rooting of cuttings during spring season, but showed poor performance or even failed to root during monsoon. Interestingly, thiamine triggered highest rooting during monsoon and was comparable during spring. Average per cent rooting was also recorded maximum in air-layers treated with thiamine (75, 150 and 300 mg l ―1 ) in comparison to auxins during both seasons. However, the number of roots per layer increased with increasing concentration of NAA and IBA in spring, but decreased in the monsoon season. The cleft grafting was found more promising in terms of success and growth of scion in the spring season. This technique can be practically applied on commercial scale in the areas where J. gossypifolia grows as a weed.