Convergence The International Journal of Research into New Media Technologies · 2001 · 20 citations · 0 references
Veterinary MedicineInfectious DiseasesMicrobiologyDiagnostic MicrobiologySequence AnalysisVeterinary Parasitic Diseases16S Ribosomal Ribonucleic Acid AnalysisMolecular Diagnosis Of ParasitesVeterinary Diagnostic MicrobiologyMaldi-tof IdentificationMaldi-tof Mass SpectrometryMass Spectrometry-based IdentificationMass Spectrometry-based Microbial IdentificationMatrix-assisted Laser Desorption/ionizationMatrix-assisted Laser Desorption/ionization Time-of-flight Mass SpectrometryReference Database ConstructionSpecies-level Microbial Identification
Capnocytophaga canimorsus and Capnocytophaga cynodegmi can be transmitted from dogs or cats and cause serious human infections. We aimed to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify these two Capnocytophaga species. Ninety-four C. canimorsus and 10 C. cynodegmi isolates identified by 16S rRNA gene sequencing were analyzed. Using the MALDI BioTyper database, correct identification was achieved for only 16 of 94 (17%) C. canimorsus and all 10 C. cynodegmi strains, according to the manufacturer's log score specifications. Following the establishment of a complementary homemade reference database by addition of 51 C. canimorsus and 8 C. cynodegmi mass spectra, MALDI-TOF MS provided reliable identification to the species level for 100% of the 45 blind-coded Capnocytophaga isolates tested. MALDI-TOF MS can accurately identify C. canimorsus and C. cynodegmi using an enriched database and thus constitutes a valuable diagnostic tool in the clinical laboratory.