Hyperoside inhibits biofilm formation of Pseudomonas aeruginosa

Abstract

<i>Pseudomonas aeruginosa (P. aeruginosa)</i> is a common pathogen in hospital-acquired infection and is readily able to form biofilms. Due to its high antibiotic resistance, traditional antibacterial treatments exert a limited effect on <i>P. aeruginosa</i> biofilm infections. It has been indicated that hyperoside inhibits <i>P. aeruginosa</i> PAO1 (PAO1) biofilm formation without affecting growth. Therefore, the current study examined the biofilm formation and quorum sensing (QS) system of PAO1 in the presence of hyperoside. Confocal laser scanning microscopy analysis demonstrated that hyperoside significantly inhibited biofilm formation. It was also observed that hyperoside inhibited twitching motility in addition to adhesion. Data from reverse transcription-quantitative polymerase chain reaction indicated that hyperoside inhibited the expression of lasR, lasI, rhlR and rhlI genes. These results suggest that the QS-inhibiting effect of hyperoside may lead to a reduction in biofilm formation. However, the precise mechanism of hyperoside on <i>P. aeruginosa</i> pathogenicity remains unclear and requires elucidation in additional studies.