Protein Extraction from Grape Seeds by Reverse Micelles: Optimization of the Forward Extraction

Abstract

The optimization of the reverse micelles extraction of protein from grape seeds was carried out using response surface methodology (RSM). Based on the Plackett-Burman design and steepest ascent, CTAB concentration, pH, NaCl concentration and crude protein concentration were selected as the most extract conditions. Subsequently, the optimum combination of the selected factors was investigated by the Box-Behnken design. The final condition of extraction optimized with RSM was CTAB concentration 39 mmol/L, pH 5.6, NaCl concentration 0.01 mol/L, and crude protein concentration 2.1 mg/mL. The forward extraction yield of 82.3% in triplicate under optimal extraction condition was obtained.