Affinity and Efficacy Studies of Tetrahydrocannabinolic Acid A at Cannabinoid Receptor Types One and Two

Abstract

<b>Introduction:</b><i>Cannabis</i> biosynthesizes Δ⁹-tetrahydrocannabinolic acid (THCA-A), which decarboxylates into Δ⁹-tetrahydrocannabinol (THC). There is growing interest in the therapeutic use of THCA-A, but its clinical application may be hampered by instability. THCA-A lacks cannabimimetic effects; we hypothesize that it has little binding affinity at cannabinoid receptor 1 (CB₁). <b>Materials and Methods:</b> Purity of certified reference standards were tested with high performance liquid chromatography (HPLC). Binding affinity of THCA-A and THC at human (h) CB₁ and hCB₂ was measured in competition binding assays, using transfected HEK cells and [³H]CP55,940. Efficacy at hCB₁ and hCB₂ was measured in a cyclic adenosine monophosphase (cAMP) assay, using a Bioluminescence Resonance Energy Transfer (BRET) biosensor. <b>Results:</b> The THCA-A reagent contained 2% THC. THCA-A displayed small but measurable binding at both hCB₁ and hCB₂, equating to approximate K_i values of 3.1μM and 12.5μM, respectively. THC showed 62-fold greater affinity at hCB₁ and 125-fold greater affinity at hCB₂. In efficacy tests, THCA-A (10μM) slightly inhibited forskolin-stimulated cAMP at hCB₁, suggestive of weak agonist activity, and no measurable efficacy at hCB₂. <b>Discussion:</b> The presence of THC in our THCA-A certified standard agrees with decarboxylation kinetics (literature reviewed herein), which indicate contamination with THC is nearly unavoidable. THCA-A binding at 10μM approximated THC binding at 200nM. We therefore suspect some of our THCA-A binding curve was artifact-from its inevitable decarboxylation into THC-and the binding affinity of THCA-A is even weaker than our estimated values. We conclude that THCA-A has little affinity or efficacy at CB₁ or CB₂.