Electrophoretic Characterization and the Relationshipbetween Some Brassica Species

Abstract

The objective of this study was to assess the genetic divergence available in different genotypes of oilseed Brassica based on analyses of seed storage proteins, for the identification of genetically diverse and agronomically superior genotypes of Brassica seed, which may generate putative transgressive segregates on hybridization. Diversity within species of Brassica can be analyzed at molecular level with the help of seed storage protein by using SDS-PAGE. On the basis of banding pattern zymogram (diagrammatical representation of different protein bands) were sketched, by which molecular weight of specie specific bands were calculated on the basis of Rf values of the bands on the gel. Through statistical analyses dendrogram was formed and genotypes were clustered into different groups by applying UPGMA (unweighed pair group mean analyses).