Kinetic Analysis of Heterotrophic Nitrification–Aerobic Denitrification by an Oligotrophic <i>Acinetobacter</i> Sp. SYF26

Abstract

Kinetics of low nitrate and ammonia concentration removal by oligotrophic Acinetobacter sp. SYF26 were examined in this study. Heterotrophic nitrification–aerobic denitrification followed first-order reaction kinetics. The maximum removal rate was achieved under pH = 7.0, 30°C, and a carbon-to-nitrogen ratio (C/N) = 7.5. In a landscape water experiment, strain SYF26 was capable of both heterotrophic nitrification and aerobic denitrification and exhibited no nitrite accumulation during the whole process. Both three-dimensional excitation–emission matrix fluorescence spectroscopy and Fourier transform near-infrared spectroscopy assessments of landscape water illustrated that strain SYF26 could use protein-like compounds and humus as carbon sources for heterotrophic nitrification–aerobic denitrification. Results illustrated that strain SYF26 is a suitable candidate for the bioremediation of landscape water.