Isolation of antimicrobial producing Actinobacteria from soil samples

Abstract

Emergence of multidrug resistant bacteria has made the search for novel bioactive compounds from natural and unexplored habitats a necessity. <i>Actinobacteria</i> have important bioactive substances. The present study investigated antimicrobial activity of <i>Actinobacteria</i> isolated from soil samples of Egypt. One hundred samples were collected from agricultural farming soil of different governorates. Twelve isolates have produced activity against the tested microorganisms (<i>S. aureus</i>, <i>Bacillus cereus</i>, <i>E. coli</i>, <i>K. pneumoniae</i>, <i>P. aeruginosa</i>, <i>S.</i> Typhi, <i>C. albicans</i>, <i>A. niger</i> and <i>A. flavus</i>). By VITEK 2 system version: 07.01 the 12 isolates were identified as <i>Kocuria kristinae</i>, <i>Kocuria rosea</i>, <i>Streptomyces griseus</i>, <i>Streptomyces flaveolus</i> and <i>Actinobacteria</i>. Using ethyl acetate extraction method the isolates culture's supernatants were tested by diffusion method against indicator microorganisms. These results indicate that <i>Actinobacteria</i> isolated from Egypt farms could be sources of antimicrobial bioactive substances.