In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications

TLDR

Bioprinting is a novel technology that can address unsolved questions in tissue engineering. The study demonstrates that laser‑assisted bioprinting can deposit mesenchymal stromal cells with collagen and nano‑hydroxyapatite to promote bone regeneration in a mouse calvaria defect model. The authors employed laser‑assisted bioprinting to print mesenchymal stromal cells, collagen, and nano‑hydroxyapatite directly into bone defects. LAB’s high resolution and precision enable in situ bone substitute printing, and varying cell geometries influence bone regeneration, opening new avenues for tissue engineering.

Abstract

Abstract Bioprinting has emerged as a novel technological approach with the potential to address unsolved questions in the field of tissue engineering. We have recently shown that Laser Assisted Bioprinting (LAB), due to its unprecedented cell printing resolution and precision, is an attractive tool for the in situ printing of a bone substitute. Here, we show that LAB can be used for the in situ printing of mesenchymal stromal cells, associated with collagen and nano-hydroxyapatite, in order to favor bone regeneration, in a calvaria defect model in mice. Also, by testing different cell printing geometries, we show that different cellular arrangements impact on bone tissue regeneration. This work opens new avenues on the development of novel strategies, using in situ bioprinting, for the building of tissues, from the ground up.