Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study

Abstract

The present study was cherished to investigate in vitro thrombolytic, membrane stabilizing and antibacterial activities of <i>Allamanda neriifolia</i> and <i>Aegialitis rotundifolia</i>. Different types methanolic extracts of these two medicinal plants were tested for determining membrane stabilizing activity at a hypotonic solution and heat induce condition by comparing with reference standard acetyl salicylic acid (0.10 mg/mL), where thrombolytic activity assessment was done by employing Streptokinase as standard drug. Finally, antibacterial activity was performed against <i>Staphylococcus aureus</i> as a Gram-positive (+ve) and <i>Salmonella typhi</i>, <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> as Gram negative (-ve) bacteria by using disc diffusion method. In case of membrane stabilizing studies, crude methanolic extracts of <i>A. neriifolia</i> at 10 mg/ml concentration, more importantly, showed 45.80% & 23.52% whereas 10 mg/ml concentration of <i>A. rotundifolia</i> more significantly (p < 0.01) produced 38.40% and 27.04% inhibition of hemolysis for both experimental conditions. Dose-dependently increased activity was found in the thrombolytic study where 10 mg/ml concentration of both <i>A. neriifolia</i> and <i>A. rotundifolia</i> more significantly (p < 0.01) showed 41.91% and 32.76% lysis of clot respectively by in vitro clot lysis assay method. Crude methanolic extracts of <i>A. rotundifolia</i> did not show any suitable antibacterial property against the test bacteria. However, the gram positive (+ve) bacteria also seemed resistant against <i>A. neriifolia</i> extract but this crude methanolic extracts was found to generate moderate antibacterial action against gram-negative (-ve) bacteria. The obtained results confirmed the presence of thrombolytic, membrane stabilizing activity for both plant extract along with moderate antibacterial activity for <i>A. neriifolia</i>.