Aggressive behaviours, food deprivation and the <i>foraging</i> gene

Abstract

A pleiotropic gene governs multiple traits, which might constrain the evolution of complexity due to conflicting selection on these traits. However, if the pleiotropic effect is modular, then this can facilitate synergistic responses to selection on functionally related traits, thereby leveraging the evolution of complexity. To understand the evolutionary consequence of pleiotropy, the relation among functionally different traits governed by the same gene is key. We examined a pleiotropic function of the <i>foraging</i> (<i>for</i>) gene with its rover and sitter allelic variants in fruit fly, <i>Drosophila melanogaster</i>. We measured <i>for</i>'s effect on adult male aggressive behaviours and whether this effect was shaped by <i>for</i>'s known role in food-related traits. Rover exhibited higher levels of offensive behaviour than sitters and s2, a sitter-like mutant on rover genetic background. With a Markov chain model, we estimated the rate of aggression escalation, and found that the rover pattern of aggressive escalation more rapidly intensified fights. Subsequent analysis revealed that this was not caused by <i>for</i>'s effect on food-related traits, suggesting that <i>for</i> might directly regulate aggressive behaviours. Food deprivation did not elevate aggression, but reduced intermediate-level aggressive behaviours. Aggression and other foraging-related behaviour might comprise a synergistic trait module underlaid by this pleiotropic gene.