Complete genome sequence and comparative analysis of the metabolically versatile <i>Pseudomonas putida</i> KT2440

TLDR

Pseudomonas putida is a metabolically versatile saprophytic soil bacterium certified as a biosafety host for foreign genes and holds significant biotechnological potential. The 6.18‑Mb genome of KT2440 encodes diverse transport and metabolic systems, shares 85 % of coding regions with the pathogenic P.

Abstract

Summary Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non‐pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.