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Handbook of research design and social measurement
236
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1993
Year
Tissue EngineeringSocial IndicatorBone RegenerationPeptide EngineeringBone RepairSocial InfluenceBiomedical EngineeringSocial SciencesRegenerative MedicineSynthetic Bone SubstituteBone Morphogenic ProteinSocial MeasurementFunctional Tissue EngineeringAsymmetric Sis MembraneCell EngineeringCell BiologyRecombinant PeptidesApplied Social ScienceSociologyQuantitative Social Science ResearchMedicineExtracellular Matrix
Guided bone regeneration (GBR) technology is an effective method for reconstructing bone defects, and barrier membranes are widely used in this process. The objective of this study was to develop a novel asymmetric SIS (small intestinal submucosa) membrane and modify the membrane with exosomes and engineered recombinant peptides. The asymmetric SIS membrane was developed with liquid nitrogen quencher. The engineered recombinant peptides were designed by connecting collagen binding peptides with the exosomal capture peptide CP05 via linker. In vitro experiments demonstrated that the engineered recombinant peptides contributed to promoting the positive effect of exosomes on the osteogenic differentiation of BMSCs. Further mechanistic studies confirmed that the PI3K/Akt signaling pathway is critical in the exosome-induced osteogenic differentiation of BMSCs. Moreover, the asymmetric SIS membrane combining engineered recombinant peptides and exosomes could reconstruct bone defects effectively within 12 weeks. Therefore, the membrane developed in this study has the potential to repair bone defects.