A Novel Biomimetic Hydrogen Peroxide Biosensor Based on Pt Flowers‐decorated Fe₃O₄/Graphene Nanocomposite

Abstract

Abstract A sensitive and selective amperometric H 2 O 2 biosensor was obtained by utilizing the electrodeposition of Pt flowers on iron oxide‐reduced graphene oxide (Fe 3 O 4 /rGO) nanocomposite modified glassy carbon electrode (GCE). The morphology of Fe 3 O 4 /rGO and Pt/Fe 3 O 4 /rGO was characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), respectively. The step‐wise modification and the electrochemical characteristics of the resulting biosensor were characterized by cyclic voltammetry (CV) and chronoamperometry methods. Thanks to the fast electron transfer at the Pt/Fe 3 O 4 /rGO electrode interface, the developed biosensor exhibits a fast and linear amperometric response upon H 2 O 2 . The linear range of Pt/Fe 3 O 4 /rGO is 0.1∼2.4 mM (R 2 =0.998), with a sensitivity of 6.875 μA/mM and a detection limit of 1.58 μM (S/N=3). In addition, the prepared biosensor also provides good anti‐interferent ability and long‐term stability due to the favorable biocompatibility of the electrode interface. The proposed sensor will become a reliable and effective tool for monitoring and sensing the H 2 O 2 in complicate environment.