A two-photon fluorescent probe for specific detection of hydrogen sulfide based on a familiar ESIPT fluorophore bearing AIE characteristics

Abstract

Hydrogen sulfide (H₂S) is a signaling gasotransmitter, which plays various roles in modulating the functions of different systems. In this study, a "turn-on" fluorescent probe for H₂S was developed. The probe, whose design is based on a familiar excited state intramolecular proton transfer (ESIPT) fluorophore bearing aggregation-induced emission (AIE) characteristics, was found to exhibit an 80-fold fluorescence enhancement along with a large Stokes shift upon the addition of H₂S. Furthermore, the probe was successfully utilized to detect H₂S in HeLa cells with both one-photon and two-photon microscopy.