Development of an efficient glucosinolate extraction method

Abstract

We present a simplified method for extracting glucosinolates from plant tissues which does not require the use of a freeze drier or boiling methanol, and is therefore less hazardous, and more time and cost effective. The presented method has been shown to have comparable or improved glucosinolate extraction efficiency relative to the commonly used ISO method for major glucosinolates in the Brassicaceae species studied: sinigrin and gluconasturtiin in <i>B. juncea</i>; sinalbin, glucotropaeolin, and gluconasturtiin in <i>S. alba</i>; glucoraphenin and glucoraphasatin in <i>R. sativus</i>; and glucosatavin, glucoerucin and glucoraphanin in <i>E. sativa</i>.