Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues

Abstract

This sensitive, reproducible, easy to do method of quantifying polyP will be a useful tool for investigation of polyP biology in mammalian cells and tissues. Although the protocol was developed for mammalian tissues, this method should be able to quantify polyP in most biological sources, including fluid samples such as blood and serum.