Annals of biological research · 2011 · 46 citations · 0 references
Life SciencesDermatologyMicrobiologyDermatopathologyMycologySkin Biopsy InterpretationFungal PathologySkin InfectionsTinea PedisAgarClinical Mycological DiagnosisDermatophytosis DiagnosisFungal DermatosesFungal Skin DiseasesMicroscopyMycological Culture TechniquesTinea Corporis
100 clinically suspected cases of dermatophytosis were subjected to mycological examination with microscopy and culture using 10% KOH and Sabouraud’s Dextrose Agar ( SDA) and Dermatophyte test medium(DTM) .Causative agents were identified macroscopically and microscopically from the growth obtained from SDA. Direct microscopy revealed fungal elements in 43% cases , Of which 58% were positive on culture. Commonest age group affected is between 21-30 years and males outnumbered females 1.3:1. 32% gave history of possible sources of infection. Tinea corporis was the commonest lesion (27% ) followed by Tinea pedis (17%) and Tinea unguium (17%) lesions in mixed sites are seen in 9% cases .Tricophyton rubrum was the commonest aetiological agent (51.72%), followed byT.mentagrophytes(31.0%), T.violaceum(6.89%), T.verrucosum (3.4%)and Microsporum gypseum (6.89). DTM is more useful as a general screening medium as opposed to an identification medium in the isolation of dermatophytes.