LC–MS/MS Simultaneous Analysis of Allopregnanolone, Epiallopregnanolone, Pregnanolone, Dehydroepiandrosterone and Dehydroepiandrosterone 3-Sulfate in Human Plasma

Abstract

For the first time, a straightforward and reliable method for the chromatographic separation of allopregnanolone, epiallopregnanolone and pregnanolone, as well as of dehydroepiandrosterone and dehydroepiandrosterone 3-sulfate was carried out, with optimal accuracy, sensitivity and specificity.