In vivo analgesic, anti-inflammatory potential in Swiss albino mice and in vitro thrombolytic activity of hydroalcoholic fruits extract from Daemonorops robusta Warb

Abstract

The analgesic, anti-inflammatory and thrombolytic potential of Daemonorops robusta Warb, a Bangladeshi tribal medicinal plant was studied for the first time. So our aim was to evaluate analgesic, antiinflammatory and thrombolytic activities of fruit extract of D. robusta. Methods: Fresh fruits of plant were extracted with methanol and then subjected to preliminary phytochemical screening. Analgesic activity of the extract was performed against acetic acid induced writhing, hot plate and formalin induced test. Anti-inflammatory effect was evaluated using carrageenan induced paw edema in mice. An In Vitro thrombolytic model was used to check the clot lysis potential of the extract. Results: Phytochemical screening showed the presence of alkaloid, carbohydrate, flavonoids, steroid, phlobatanin and saponin. The extract exhibited significant analgesic effect ( P<0.001) at dose-dependent manner. In addition, administration of glibenclamide, an ATP-sensitive K + channel antagonist partly reversed antinociceptive activity shown by the plant extract. It is observed that the anti-nociceptive activity of plant extract involves the NO-cGMP pathway. Besides, the anti-inflammatory activity of plant extract was significant (P<0.001). In thrombolytic activity assay, a moderate percentage (%) of clot lysis was observed when compared to standard drug streptokinase. Conclusion: Our findings suggest that the fruits of D. robusta possess significant analgesic and antiinflammatory activities. This plant also contains moderate thrombolytic potential.