Proceedings of the National Academy of Sciences · 2017 · 88 citations · 49 references
Life SciencesPlant BiologyPlant SciencesPlant BiotechnologyPlant Molecular BiologyRna ProcessingPlant EnzymesPlant Small Rna BiologyRna CleavageRna Processing In PlantsChloroplast Rna ProcessingEndonucleolytic Rna CleavagePentatricopeptide Repeat ProteinsPlastid Rna ProcessingThylakoid Biogenesis
pentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has RNA endonuclease activity. We show that the SMR moiety of SOT1 performs the endonucleolytic maturation of 23S and 4.5S rRNA through the PPR domain, specifically recognizing a 13-nucleotide RNA sequence in the 5' end of the chloroplast 23S-4.5S rRNA precursor. In addition, we successfully engineered the SOT1 protein with altered PPR motifs to recognize and cleave a predicted RNA substrate. Our findings point to SOT1 as an exciting tool for RNA manipulation.
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Signals from chloroplasts converge to regulate nuclear gene expression.
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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
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