Seroepidemiological Analysis of Contagious Caprine Pleuropneumonia through cELISA in Selected Districts of Khyber Pakhtunkhwa-Pakistan

Abstract

Contagious caprine pleuropneumonia (CCPP) is a fatal disease of goats caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp).This disease has been causing huge economic losses to goat rearing farmers in Khyber Pakhtunkhwa.  Seroepidemiological study of this disease was conducted for the first time in selected districts of Khyber Pakhtunkhwa namely Swat, Peshawar, Kohat and Dera Ismail Khan. Total 384 serum samples were collected randomly from goats having different ages and both sexes showing respiratory signs belonging to flocks with no vaccination history against CCPP. The serum samples were examined for Mccp directed antibodies using monoclonal antibody based cELISA. Out of total 384 samples 15 samples were detected positive on cELISA with 3.91% overall seroprevalence in the selected districts. The highest CCPP seroprevalence was recorded in district Swat (8.33%) followed by district Kohat and D.I Khan (3.13% in each district) and the lowest seroprevalence was observed in district Peshawar (1.04%). Age based CCPP seroprevalence was found highest (6.73%) in the goat kids of age 1 to 180 days followed by the (3.85%) young goat of 181 to 365 days while the lowest was found in the adult goats (2%) with age more than one year. Sex based seroprevalence was found more in female goats (4.01%) as compare to male goats (3.33%). This study determined the load of CCPP using highly specific monoclonal antibody based cELISA kit in the selected districts of Khyber Pakhtunkhwa for the first time. Further study on CCPP is needed with increased sample size, that cover wider region of Khyber Pakhtunkhwa as necessary for limiting this disease through effective control measures.