Optics Letters · 2017 · 16 citations · 18 references
EngineeringBioengineeringBiomedical EngineeringBiomedical ImagingBiomedical OpticsDiagnostic ImagingHemodynamicsLabel-free ImagingOptical DiagnosticsTissue Optical PropertiesTissue OpticsVascular ImagingBlood FlowBlood Flow MeasurementFlow Velocity EstimationFlow Velocity MeasurementsIntensity ModulationInterferenceLow-coherence InterferometryReal-time Contrast Imaging
We propose full-field functional optical angiography for a live biological specimen based on the absorption intensity fluctuation modulation (AIFM) effect. Because of the difference in absorption between red blood cells (RBCs) and the background tissue under low-coherence light illumination, the moving RBCs, which discontinuously pass though the capillary vessels, generate an AIFM effect. This effect offers high contrast of absorption imaging and sensitivity of low-coherence interference between RBCs and the background tissue. It is used to distinguish the signal of RBCs from that of the background tissue. The averaged and real-time modulation depths are computed to obtain full-field label-free optical angiography and measure blood flow velocity simultaneously. The AIFM method could potentially be applied to study the physiological mechanisms of blood circulation systems of near-transparent live biologic samples.
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Optical Constants of Water in the 200-nm to 200-μm Wavelength Region
George M. Hale, Marvin R. Querry · Applied Optics · 1973 · 4.4K citations
Three dimensional optical angiography
Ruikang K. Wang, Steven L. Jacques, Zhenhe Ma et al. · Optics Express · 2007 · 632 citations · Full text