MORPHOLOGICAL CHARACTERISTICS OF FOUR Trichoderma ISOLATES AND TWO ENDOPHYTIC Fusarium ISOLATES

Abstract

The aim of this research was to know the exact morphological characteristics of some antagonistic microbes. The research was carried out at the Laboratory of Microbiology, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. The morphological characteristic was done by observation of fungal colony growth on PDA and MEA media, on several nutrition such as nitrogen and carbon, pigmentation observation, and fungal colony growth at various temperatures. Result of the research showed that the antagonistic fungi of Trichoderma spp. and the endophytic Fusarium isolates had specific morphological structure that are commonly similar for the same species and could be identified as T. harzianum , T. pseudokongii , and F. oxysporum but conidia size and mycelial wet or dry weight were different and gave different growth on PDA and MEA but better growth on PDA. Trichoderma harzianum could grow on Czapek and gelatin media, while endophytic Fusarium oxysporum and T. pseudokoningii could grow on Czapek, nitrit agar, and gelatin media. The temperature range for growth of T. harzianum and T. pseudokongii was 20-35oC with the optimum at 30oC.