Anti-inflammatory activity of manassantin A from ultra-fine ground Saururus chinensis in lipopolysaccharide-stimulated RAW 264.7 cells

Abstract

As a research of inflammation inhibitory activity using a natural resource, the inflammation inhibitory activity by purified manassantin A from Saururus chinensis was experimented. In the result of MTT assay with manassantin A, cell viability decreased at concentration of 100 μM. LPS-treated RAW 264.7 cell group treated with 6.25–50 μM concentration of manassantin A showed approximately 4–55% NO expression compared to LPS non-treated group. Inflammation inhibitory activity and NO expression inhibition increased as RAW 264.7 cell treated with higher concentration of manassantin A. Expression inhibition of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was also identified. An excellent prostaglandin E 2 expression inhibition effect was identified with manassantin A at concentration of 6.25–25 μM. A high interleukin-1β, tumor necrosis factor-α, and IL-6 expression inhibition effect was manassantin A at concentration of 6.25–50 μM of 0–97, 6–32, and 22–66% was identified, respectively. A high interleukin-1β, tumor necrosis factor-α, and IL-6 expression inhibition effect was manassantin A at concentration of 6.25–50 μM of 0–97, 6–32, and 22–66% was identified, respectively. Expression inhibition effect was identified to be concentration dependent. Therefore, manassantin A is expected to show high inflammation inhibitory effect in RAW 264.7 induced from LPS by inhibiting iNOS and COX-2 protein expression along with cytokine expression inhibition.