Temperature response of mesophyll conductance in three C₄ species calculated with two methods: ¹⁸O discrimination and <i>in vitro V</i>_pmax

Abstract

Mesophyll conductance (g_m ) is an important factor limiting rates of C₃ photosynthesis. However, its role in C₄ photosynthesis is poorly understood because it has been historically difficult to estimate. We use two methods to derive the temperature responses of g_m in C₄ species. The first (Δ¹⁸ O) combines measurements of gas exchange with models and measurements of ¹⁸ O discrimination. The second method (in vitro V_pmax ) derives g_m by retrofitting models of C₄ photosynthesis and ¹³ C discrimination with gas exchange, kinetic constants and in vitro V_pmax measurements. The two methods produced similar g_m for Setaria viridis and Zea mays. Additionally, we present the first temperature response (10-40°C) of C₄ g_m in S. viridis, Z. mays and Miscanthus × giganteus. Values for g_m at 25°C ranged from 2.90 to 7.85 μmol m^-2 s^-1 Pa^-1 . Our study demonstrated that: the two described methods are suitable to calculate g_m in C₄ species; g_m values in C₄ are similar to high-end values reported for C₃ species; and g_m increases with temperature analogous to reports for C₃ species and the response is species specific. These results improve our mechanistic understanding of C₄ photosynthesis.