Fluorescence detection of endogenous bisulfite in liver cancer cells using an effective ESIPT enhanced FRET platform

Abstract

Probe L-HF1, which featured large (pseudo) Stokes shifts and high FRET efficiency, was designed on a new ESIPT enhanced FRET platform for the detection of HSO₃^-/SO₃^2-. L-HF1 could detect endogenous bisulfite in HepG2 cells but not in L-02 cells, implying the different bisulfite levels in normal and cancer cells of liver.