Identification of<i>ITGA2B</i>and<i>ITGB3</i>Single-Nucleotide Polymorphisms and Their Influences on the Platelet Function

Abstract

The aim of the study was to investigate <i>ITGA2B</i> and <i>ITGB3</i> genetic polymorphisms and to evaluate the variability in the platelet function in healthy Chinese subjects. The genetic sequence of the entire coding region of the <i>ITGA2B</i> and <i>ITGB3</i> genes was investigated. Adenosine diphosphate-induced platelet aggregation, glycoprotein IIb/IIIa content, bleeding time, and coagulation indexes were detected. Thirteen variants in the <i>ITGA2B</i> locus and 29 variants in the <i>ITGB3</i> locus were identified in the Chinese population. The rs1009312 and rs2015049 were associated with the mean platelet volume. The rs70940817 was significantly correlated with the prothrombin time. The rs70940817 and rs112188890 were related with the activated partial thromboplastin time, and <i>ITGB3</i> rs4642 was correlated with the thrombin time and fibrinogen. The minor alleles of rs56197296 and rs5919 were associated with decreased ADP-induced platelet aggregation, and rs55827077 was related with decreased GPIIb/IIIa per platelet. The rs1009312, rs2015049, rs3760364, rs567581451, rs7208170, and rs117052258 were related with bleeding time. Further studies are needed to explore the clinical importance of <i>ITGA2B</i> and <i>ITGB3</i> SNPs in the platelet function.