Identification of Staphylococcus aureus and Coagulase Negative Staphylococci Isolates from Mastitis Milk of Etawa Crossbred Goat

Abstract

This study aimed to identify staphylococcal mastitis milk in Peranakan Etawa (PE) or Etawa crossbred goats in Indonesia. Total of 93 milk samples used in this study, there were 49 (52.7%) subclinical and 3 (3.2%) clinical mastitis samples. From 52 subclinical and clinical mastitis samples could be isolated 20 (38.5%) staphylococci. Phenotypic identification were performed based on the Gram staining, Mannitol Salt Agar (MSA), catalase, Voges-Proskauer (VP) and coagulase tests. Molecular identification were determined using the Polymerase Chain Reaction (PCR) to detect several genes such as 23S rRNA, nuc and coa genes and confirmed with DNA sequencing. Based on phenotypic and genotypic identification, could be identified 10 Coagulase Positive Staphylococci (CPS) indicated Staphylococcus aureus and 10 Coagulase Negative Staphylococci (CNS) isolates, including Staphylococcus pasteuri (3 isolates), Staphylococcus xylosus (5 isolates) and Staphylococcus haemolyticus (2 isolates). From 3 clinical mastitis of PE goats in our study could be isolated three different staphylococci i.e., S. aureus, S. pasteuri and S. xylosus. This finding indicated that both CPS and CNS have important role as causative pathogen of mastitis in PE goats.