Anti-fungal Activity of Bioconverted Oil Extract of Linoleic Acid and Fractionated Dilutions against Phytopathogens Rhizoctonia solani and Botrytis cinerea

Abstract

Oil extract, bioconverted from linoleic acid by a bacterial strain Pseudomonas aeruginosa PR3, and its stepwise chromatographically fractionated dilutions were chemically analyzed by gas chromatography (GC) and thin layer chromatography (TLC), and evaluated for their anti-fungal activities against Botrytis cinerea and Rhizoctonia solani. The extract sample () showed high anti-fungal activity against R. solani (55%) and B. cinerea (53%) as compared to fractionated dilutions. Especially the fractionated dilutions of F2 (60 : 40) showed higher anti-fungal activities against B. cinerea (53%) as compared to R. solani (49%). Rest of fractionted dilutions showed relatively low anti-fungal activities against both the strains R. solani and B. cinerea (F1: 39%-43%, F3: 39%-47%, F4: 17%-49%, F5: 42%-50%) respectively. Oil extract sample showed anti-fungal activity with minimum inhibitory concentration (MIC), ranging from 500 to . The growth inhibition percentages of oil extract of bioconverted linoleic acid and fractionated dilutions were measured as morphological abnormalities for both R. solani and B. cinerea involving lysis, distortion and swelling in hyphae, and also the screening was carried out using varied oil extract concentrations for the determination of antifungal effects on the spore germination of B. cinerea.