Role of O ₂ in the Growth of Rhizobium leguminosarum bv. viciae 3841 on Glucose and Succinate

Abstract

Rhizobium leguminosarum, a soil bacterium that forms N₂-fixing symbioses with several agriculturally important leguminous plants (including pea, vetch, and lentil), has been widely utilized as a model to study Rhizobium-legume symbioses. Insertion sequencing (INSeq) has been used to identify factors needed for its growth on different carbon sources and O₂ levels. Identification of these factors is fundamental to a better understanding of the cell physiology and core metabolism of this bacterium, which adapts to a variety of different carbon sources and O₂ tensions during growth in soil and N₂ fixation in symbiosis with legumes.