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The N-hydroxylation of phentermine (2-methyl-2-amino-1-phenylpropane). Properties of the enzyme system.
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1974
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Chemical BiologyEnzymatic ModificationGas Chromatographic ProcedureBioanalysisToxicologyHepatotoxicityClinical ChemistryStructure-function Enzyme KineticsHuman MetabolismChromatographyLiver Microsome PreparationsBiochemistryBiocatalysisLiver PhysiologyExperimental ToxicologyPharmacologyDrug-induced Liver InjuryBiomolecular EngineeringNatural SciencesEnzyme CatalysisEnzyme SpecificityEnzyme SystemMetabolismMedicinePharmacokineticsCarbon MonoxideDrug Analysis
A gas chromatographic procedure for the estimation of N-hydroxyphentermine (2-methyl-2-hydroxyamino-1-phenylpropane) has been used to characterize the enzyme system present in liver microsome preparations that effects the N-hydroxylation of phentermine (2-methyl-2-hydroxyamino-1-phenylpropane) in vitro . When rabbit liver microsomes were used, the reaction was inhibited by SKF 525-A and by carbon monoxide. The rabbit microsomal preparation was unstable and decayed to 50% of original activity after 10 days of storage at -70°C. The relative order of N-hydroxylation activity in the three species examined was rabbit > guinea pig ≥ rat. Kinetic studies were also conducted.