Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction

Abstract

This is the first report on limonene biosynthesis in oleaginous yeast <i>Y. lipolytica</i> by heterologous expression of codon-optimized <i>tLS</i> and <i>tNDPS1</i> genes. To our knowledge, the limonene production 23.56 mg/L, is the highest limonene production level reported in yeast. In short, we demonstrate that <i>Y. lipolytica</i> provides a compelling platform for the overproduction of limonene derivatives, and even other monoterpenes.