The Journal of Cell Biology · 2016 · 23 citations · 30 references
Molecular RegulationMolecular BiologyCytoskeletonCell CycleCell RegulationAutophagyProteomicsProtein DegradationCell SignalingMt PolymerizationSpindle DynamicsProtein FunctionCell DivisionProper Dynamic RegulationTranslational ProteomicsCell BiologyChromatinProteasomal DegradationNatural SciencesGenome IntegritySystems BiologyMedicine
Proper dynamic regulation of the spindle is essential for successful cell division. However, the molecular mechanisms that regulate spindle dynamics in mitosis are not fully understood. In this study, we show that Cullin 5-interacting suppressor of cytokine signaling box protein ASB7 ubiquitinates DDA3, a regulator of spindle dynamics, thereby targeting it for proteasomal degradation. The presence of microtubules (MTs) prevented the ASB7-DDA3 interaction, thus stabilizing DDA3. Knockdown of ASB7 decreased MT polymerization and increased the proportion of cells with unaligned chromosomes, and this phenotype was rescued by deletion of DDA3. Collectively, these data indicate that ASB7 plays a crucial role in regulating spindle dynamics and genome integrity by controlling the expression of DDA3.
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Rbx1, a Component of the VHL Tumor Suppressor Complex and SCF Ubiquitin Ligase
Takumi Kamura, Deanna M. Koepp, Michael N. Conrad et al. · Science · 1999 · 783 citations
Twenty proteins containing a C-terminal SOCS box form five structural classes
Douglas J. Hilton, Rachael T. Richardson, Warren S. Alexander et al. · Proceedings of the National Academy of Sciences · 1998 · 743 citations
Protein Assembly, Biomolecular Structure Prediction, Structural Bioinformatics +25