Histopathological evaluation of the toxic effects of zinc oxide (ZnO) nanoparticles on testicular tissue of NMRI adult mice

Abstract

In this study, the histopathological effect of zinc oxide nanoparticles (ZnOn) on testicular tissue of mature NMRI mice was investigated. Zinc oxide nanoparticles have various applications in industry and medicine because of their small size and high surface area. The animals were divided into five groups randomly=6. The first group (group1) was treated with ZnOn with a dose of 250 mg/kg/day dissolved in 1ml of distilled water. All injections were intraperitoneal (IP) for one day. The experimental 2 group (group 2) was treated with ZnOn with a dose of 500 mg/kg/day. The experimental group 3 (group 3) was treated with ZnOn with a dose of 700 mg/kg/day. The sham group (group 4) received only the same volume of distilled water (1ml) by IP injection during the experimental period. The control group (group 5) did not receive any solution. The results revealed significant changes in cell types of testis tissue that were treated with ZnO nanoparticle. These changes were observed as reduction and loss of cells in seminiferous tubules in testicular tissue of experimental groups. Thus according to the findings of this study, ZnO nanoparticles have destructive effects on testis tissue and effects on spermatogenesis.