Characterization of<i>Pectinatus Cerevisiiphilus</i>and<i>P. Frisingensis</i>by Ribotyping

Abstract

A total of 39 Pectinatus strains have been ribotyped in this investigation. Thirteen different ribotypes were observed from 34 strains of P. frisingensis, while three different types were shown with five strains of P. cerevisiiphilus when digested with EcoRI. These patterns consisted of two series of fragments: fragments conserved within a species, and polymorphic variation. P. frisingensis had conserved fragments of ≈2.5– 3, 7–8, and 10–20 kbp, whereas P. cerevisiiphilus had ≈2–3, 5–6, and 20– 30 kbp. These fragment patterns, characterized by sets of conserved fragments within a species, were species specific. Furthermore, the HindIII-and BamHI-digested restriction patterns have been examined to facilitate differentiation at the strain level. Thirty-four strains of P. frisingensis could be divided into 1 of 12 types. On the other hand, a composite ribotype, which combined the ribotypes produced by all restriction enzymes used, produced such high definition that all strains of P. frisingensis fell into 1 of 17 types.