The expression level of SPRY4-IT1 was significantly higher in glioma in comparison to normal matched tissue (p < 0.01). Furthermore, lncRNA SPRY4-IT1 was associated significantly with WHO grade (p = 0.009) and tumor size (p = 0.003). A significant difference was found that glioma patients with high SPRY4-IT1 expression level had distinctly shorter OS than patients with low SPRY4-IT1 expression level. Furthermore, Multivariate analysis indicated SPRY4-IT1 as an independent prognostic indicator for glioma patients (p = 0.003), CONCLUSIONS: The lncRNA SPRY4-IT1 may be a potential prognostic bio¬marker of glioma.