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Response of Dental Pulp Stem Cells to Synthetic, Allograft, and Xenograft Bone Scaffolds
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2016
Year
Tissue EngineeringEngineeringBone RepairTissue TransplantationBiomedical EngineeringCell DifferentiationXenograft Bone ScaffoldsRegenerative MedicineSynthetic Bone SubstituteRegenerative BiomaterialsTranslational Tissue EngineeringAutologous TissueTissue RepairAppropriate ScaffoldTooth DevelopmentFunctional Tissue EngineeringCell BiologyBone MetabolismClinical SuccessDental RegenerationStem Cell ResearchStem-cell TherapyHard Tissue EngineeringMedicine
Different degrees of clinical success have been reported for synthetic, allograft, and xenograft bone substitutes in human trials. Although these substitutes have been clinically investigated, their in vitro effects on cell differentiation remain unclear. Proliferation, differentiation, and attachment of dental pulp stem cells (DPSCs) to β-tricalcium phosphate (β-TCP), freeze-dried bone allograft (FDBA), and deproteinized bovine bone mineral (DBBM) were compared in this study. MTT assay, measurement of total DNA, and reverse transcriptase polymerase chain reaction were performed. β-TCP had the highest potential for DPSC attachment and proliferation, while FDBA induced osteoblastic differentiation of DPSCs. Further in vivo investigations are necessary to select a clinically appropriate scaffold.