2011 · 22 citations · 6 references
AgriculturePlant BiologyPlant SciencesCrop ManagementCrop ProductionPlant PropagationGermplasm ConservationGermplasm EnhancementIn Vitro ConservationMedicinal Plant ProductionPlant GrowthPlant Growth RegulationConservation Biology Of Medicinal PlantsConservation Of Medicinal PlantsCultivation Of Medicinal PlantsEx Vitro EstablishmentMedicinal Plant ConservationMicropropagationPlant Growth Regulator UseSustainable Use Of Medicinal Plants
Coleus forskohlli Briq., an endangered medicinal plant, has been successfully micropropagated under in vitro conditions. Best adventitious shoots were developed in solid Murashige and Skoog’s medium supplemented with 1.5 mg L 6-benzylaminopurine, while best rhizogenesis was achieved without growth regulator. The second event of success was in vitro conservation of the species by slow growth technique employing osmotic regulators (sorbitol and mannitol). 3 M mannitol showed best performance for reducing growth. This study elucidated an efficient method of ex situ conservation of germplasm and sustainable management of this medicinally important plant.
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