Identification of Vitis vinifera L. × Muscadinia rotundifolia Small Hybrids by Starch Gel Electrophoresis

Abstract

Abstract Four isozyme systems, glucosephosphate isomerase (PGI, EC 5.3.1.9), phosphoglucomutase (PGM, EC 2.7.5.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), and malate dehydrogenase (MDH, EC 1.1.1.37), were identified as useful in detecting intergeneric Vitis vinifera × Muscadinia rotundifolia hybrids. Polymorphism between and within the two genera was observed at the PGI-2, PGM-2, and IDH-1 loci. However, the two appeared fixed for different alleles at the MDH-3 locus. The combination of any two of the enzyme systems allowed for rapid identification of F 1 hybrids at the young, pre-fruiting seedling stage.