Recurrent Selection for Resistance to White Mold in Phaseolus Species

Abstract

Abstract Recurrent selection was used to breed Phaseolus species for resistance to white mold disease, caused by Sclerotinia sclerotiorum . Twenty diverse genotypes selected for resistance to white mold formed the cycle 0 population. These lines were intercrossed in a partial diallel, and the F 2 progeny were tested for resistance to white mold using a detached blossom/ascospore technique. Twenty single-plant selections were made, and the F 3 progeny of these selections formed the cycle 1 population. A 2nd cycle of intercrossing, evaluation, and selection was completed, and genetic gain was determined by evaluating disease resistance of the selfed progeny derived from the cycle 0, 1, and 2 populations. There was a highly significant linear improvement in mean disease response from cycle 0 to cycle 2. When a subjective rating was used to assess disease response, the average gain/cycle was 0.52 rating units, and the percentage gain from cycle 0 to cycle 2 was 31%. When measurement of lesion length (centimeters) on the main stem was used to assess disease response, the average gain/cycle was 1.96 cm, and the percentage gain from cycle 0 to cycle 2 was 50%. The results of this study indicate that recurrent selection may be a useful technique for the development of resistance to white mold in Phaseolus species.